The University of Maryland, Baltimore County is the first-ever 16-seed to beat a number-one seed in NCAA Tournament history.
The Retrievers stunned top-overall seed Virginia 74-54 in first round action of the South Region in Charlotte.
Entering this game, number-one seeds were 135-and-0 all-time since the field expanded to 64 teams in 1985.
Jairus Lyles dropped in a game-high 28 points for UMBC, which advances to face ninth-seeded Kansas State in the second round on Sunday.
Kyle Guy and Ty Jerome netted 15 points apiece for the Cavaliers, who finish the year 31-and-3.